ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between pemphigus vulgaris (PV) and human leukocyte antigen (HLA) in Han nation of northeast China.</p><p><b>METHODS</b>Standard microcytotoxicity test and polymerase chain reaction-sequence specific primers method were used to detect the HLA class I antigens and HLA-DRB1 and DQB1 alleles in 27 patients with PV and results were compared with control group.</p><p><b>RESULTS</b>Gene and phenotype frequencies of HLA-A3, A26(10), B60(40), and B13 (27.99%, 48%; 16.11%, 30%; 23.02%, 41%; 16.11%, 30%, respectively) increased significantly in PV group compared with control (1.01%, 2%; 0.5%, 1%; 4.61%, 9%; 5.13%, 10%, respectively). After P value correction, the difference of A3, A26 (10), and B60 (40) between the two groups was still significant. The gene frequencies of HLA-DRB1*140x (1401, 1404, 1405, 1407, 1408), DRB1*120x, and DQB1*0503 alleles in PV group (42.26%, 25.46%, and 23.02%) were significantly higher than control group (5.09%, 7.74%, and 1.89%). After P value correction, the difference was still significant between the two groups.</p><p><b>CONCLUSION</b>PV significantly relates with HLA in PV patients of Han nation of northeast China.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Asian People , Ethnology , China , Ethnology , Gene Frequency , HLA-A Antigens , Genetics , HLA-A3 Antigen , Genetics , HLA-B Antigens , Genetics , HLA-DQ Antigens , Genetics , HLA-DQ beta-Chains , HLA-DR Antigens , Genetics , HLA-DRB1 Chains , Pemphigus , Genetics , PhenotypeABSTRACT
<p><b>OBJECTIVE</b>To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.</p><p><b>METHODS</b>Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.</p><p><b>RESULTS</b>The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Alleles , China , DNA , Genetics , Gene Frequency , Genotype , HLA-A Antigens , Genetics , HLA-B Antigens , Genetics , HLA-C Antigens , Genetics , Linkage Disequilibrium , Oligonucleotide Probes , Polymerase Chain Reaction , MethodsABSTRACT
Objective To investigate the expression of glucocorticoid receptor-alpha and beta(GR?and GR?)mRNA of peripheral blood mononuclear cells(PBMCs)in patients with dermatomyositis/polymyositis (DM/PM)and analyze their association with the pathogenesis and clinical response to glucocorticoid therapy. Methods The expression of GR?and GR?mRNA in PBMCs from 20 normal controls and 36(24 steroid- sensitive,12 steroid-resistant)patients with DM/PM were detected by reverse transcription polymerase chain reaction(RT-PCR).Results Compared to normal controls,the expression of GR?mRNA in the DM/PM was significantly decreased(P
ABSTRACT
0.05).But GR number[sites/cell]and the expression of GR mRNA in PBMCs from PM/DM was significantly lower than those in healthy controls(P